This  work  sought  to  verify  the  presence  of  compounds  with  antimicrobial  properties  in  extracts  of
ectomycorrhizal  fungi  or  in  Picea  glehnii  inoculated  with  ectomycorrhizal  fungi.  Extracts  from  Pisolithus
tinctorius, Scleroderma flavidum, Amanita pantherina and Paxillus sp., grown in liquid culture media, and from P.
glehnii seedlings inoculated or not with the above ectomycorrhizal fungi and cultivated in in vitro condition, were
processed to obtain  two  fractions, water and ethyl acetate solubles. These  fractions were tested  for the presence of
inhibitory  constituents  against  Fusarium  roseum,  Pythium  sp.  and  Rhizoctonia  solani.  Direct  bioautography
technique on TLC or paper disc technique was used, depending on the extract and pathogenic fungi tested. The
results showed the production on inhibitory components, not only by ectomycorrhizal fungi, but also by P. glehnii
inoculated or not with ectomycorrhizal fungi. The sensitivity varied considerably according to the type of fungus or
extract.