Screening of 12 species of  Shorea  and 2 species of  Anisoptera  from the forest of Pasir
Mayang, Jambi with brine shrimp (Anemia salina) lethality bioassay showed that Shorea gibosa and
Anisoptera marginata  have sufficient activity for further investigation. Bioassay-guided
fractionation of the active extract of  S. gibosa  led to the isolation of stigmasterol and the
shoreaphenol. Bioassay-guided fractionation of the active extract of A. marginata resulted in the
isolation of lupenone and 3-methoxy-4-hydroxybenzoic acid O-p-C glucopyranoside as the bioactive
compound.