The objective of this study was to obtain the viability and plasma membrane integrity of the spotted buffalo epididymal sperm after addition of dextrose into Andromed^Ò extender.  Spermatozoa that have been collected from cauda epididymis were diluted with Andromed^Ò extender as control (K) and Andromed^Ò + 0.2% dextrose (P1) and Andromed^Ò + 0.4% dextrose (P2) as treatments.  The results showed that the quality of epididymal spermatozoa decreased during cryopreservation process.  The percentage of motility after thawing in P1 (46%) and P2 (46.67%) were significantly higher (P<0.05) compared to K (41%) as well as the percentage of live sperm in P1 (58.8%) and P2 (60%) compared to K (52.2%). The percentage of membrane integrity in P1, P2 and K were 67.4; 66.8 and 68 %, respectively.  In conclusion, the addition of 0.2 and 0.4% of dextrose into Andromed^Ò acted as an extra cellular cryoprotectant and could maintain the viability and membrane integrity of the spotted buffalo epididymal spermatozoa after thawing.